Polarized Light Microscopy in Mammalian Oocytes
نویسندگان
چکیده
منابع مشابه
Quantitative polarized light microscopy of unstained mammalian cochlear sections.
Hearing loss is the most common sensory deficit in the world, and most frequently it originates in the inner ear. Yet, the inner ear has been difficult to access for diagnosis because of its small size, delicate nature, complex three-dimensional anatomy, and encasement in the densest bone in the body. Evolving optical methods are promising to afford cellular diagnosis of pathologic changes in t...
متن کاملPolarized light microscopy.
Polarized light is the light in which all the rays vibrate in one plane. A polarizing microscope has two disk accessories. They are made up of polarizing plastic that allows light vibrating in one plane to pass. One of them is called a polarizer (placed below the condenser). Another similar disc is placed in the top part of the microscope and cuts off all the light vibrating in a perpendicular ...
متن کاملLimited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy.
BACKGROUND Spindles are formed from microtubules and are exquisitely sensitive to changes in temperature. An orientation-independent polarized light microscope, the Polscope, can be used to image spindles in living oocytes allowing analysis of spindle kinetics in the living state. This study examined the effects of cooling on spindle disassembly in living human oocytes and spindle recovery afte...
متن کاملNotes on the applications of polarized light in microscopy
Brewster (1830a) points out that beautiful color effects will be seen if metallic salts are allowed to crystallize from solution under a microscope in polarized light. For this he recommends a glass-plate polarizer and a thin plate of agate or tourmaline as analyzer. The first one to equip a microscope with Nicol prisms was probably Talbot (1834a, 1837) who describes various observations with t...
متن کاملThe use of polarized light microscopy in IVF
Infertility is defined as the inability of a couple to conceive after 1 year of unprotected sexual intercourse. Approximately 15% of couples worldwide experience some form of infertility. The treatment of infertility with IVF has become an almost routine procedure for millions of couples experiencing infertility. The first IVF baby was conceived in 1978. By 2010 more than 4 million babies had b...
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ژورنال
عنوان ژورنال: Reproduction in Domestic Animals
سال: 2010
ISSN: 0936-6768,1439-0531
DOI: 10.1111/j.1439-0531.2010.01621.x